Coding

Part:BBa_K223036:Design

Designed by: Suzanne Bartram   Group: iGEM09_Stanford   (2009-07-05)

RALDH II gene- converts retinal into retinoic acid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 95
    Illegal BglII site found at 1229
    Illegal XhoI site found at 76
    Illegal XhoI site found at 1132
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 947


Design Notes

Source

RALDH II gene sequence taken from paper

Cloning of a cDNA Encoding an Aldehyde Dehydrogenase and Its Expression in Escherichia coli ~Xianshu Wang, Peter Penzes, and Joseph L. Napoli‡

http://www.jbc.org/cgi/reprint/271/27/16288

References